Invitrogen™ Multispecies Leukotriene B4 Competitive ELISA Kit

Description

ELISA

The Multispecies Leukotriene B4 (LTB4) ELISA quantitates LTB4 in human saliva,urine,porcine plasma and tissue culture medium.

Principle of the method

This LTB4 competitive ELISA kit is an immunoassay for the quantitative determination of leukotriene B4 (LTB4) in biological fluids. The assay is based on the competition between LTB4 in the standard or sample and alkaline phosphatase conjugated LTB4 (LTB4-AP) for a limited amount of LTB4 monoclonal antibody bound to an anti-Rabbit IgG precoated 96-well plate. As the concentration of LTB4 in the sample increases,the amount of LTB4-AP captured by the coating antibody decreases. Thus,there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Leukotriene B4 (LTB4) is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind and cross into the tissue. In neutrophils, LTB4 is a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosomal enzymes.

Specifications

• Assay Range: 11.7 to 120,000 pg/mL
• Assay Sensitivity: <5.63 pg/mL
• Description: LTB4 Competitive ELISA Kit
• Conjugate: AP
• Immunoassay Kit Format: Colorimetric ELISA (p-NPP substrate) with Plates
• Product Type: ELISA
• Sample Type: Plasma, Saliva, Supernatant, Urine
• Specificity: No significant cross-reaction with PGE2, PGF2a, 20-OH-LTB4, 20-COOH-LTB4, LTC4, LTD4, LTE4, 5(S)-HETE, 12(S)-HETE, 15(S)-HETE; less than 1% cross-reaction with 12-epi-LTB4; approx. 5% cross-reaction with 6-trans-LTB4 and 6-trans-12-epi-LTB4
• Sufficient For: 96 microplate wells
• Target Species: Human, Porcine
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 12.4%
• Intraassay CV: 6.5
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent, Detection Antibody, AP Conjugate, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: AP
• Measurement: Absorbance at 405 nm minus absorbance (at 570 nm)
• Precision: Dependent upon sample type and level
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Saliva 25 μL, Supernatant 100 μL, Urine 100 μL
• Storage Requirements: -20°C
• Target: Leukotriene B4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov