Applied Biosystems™ ViiA™ 7 Array Card Spectral Calibration Kit

Catalog No.	Quantity
44-323-14	1 kit

Catalog No. 44-323-14 Supplier Applied Biosystems™ Supplier No. 4432314

Description

Includes

Empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC™ dye, ROX™ dye, ROI dye mix, background buffer, FAM/ROX dye, VIC/ROX dye

Perform spectral calibration, region-of-interest (ROI) calibration and normalization on the ViiA™ 7 or QuantStudio™ 7 Flex or 12K Flex systems with array card block. Applied Biosystems™ ViiA™ 7 Array Card Spectral Calibration Kit includes empty array cards and seven tubes of calibration dyes.

It includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC™ dye, ROX™ dye, ROI dye mix, background buffer, FAM™/ROX™ dye, VIC™/ROX™ dye. Please see your instrument maintenance guide for full instructions on use. The dye formulations for your ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

The Applied Biosystems™ ViiA™ 7 Array Card Spectral Calibration Kit includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC™ dye, ROX™ dye, ROI dye mix, background buffer, FAM™⁄ROX™ dye, VIC™⁄ROX™ dye.

The dye formulations for the ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in real-time PCR experiments.

Order Info

Store plates at -15° to -25°C

Specifications

• Format: 384-well Microfluidic Card
• Content And Storage: Store 7 Tubes of Calibration Dyes at -15°C to -25°C
• Calibrated Dye: FAM, ROX, VIC
• For Use With (Equipment): QuantStudio™ 12 K Flex, QuantStudio™ 7, ViiA™ 7 System
• Product Line: ViiA
• Product Type: Array Card Spectral Calibration Kit
• For Use With (Application): Real Time PCR (qPCR)
• Quantity: 1 kit
• Calibration Type: Spectral Calibration, Region-of-Interest (ROI) Calibration, Normalization

Frequently Asked Questions (FAQs)

I cannot open the QuantStudio 3/5 Design & Analysis 2 (DA2) software on my Mac. What are the software requirements for Mac?

To open the software download on your Mac, you will need to adjust your security settings using the following instructions from Apple support:

Safely open apps on your mac

Please note, the software should run on Mac OS 10.10 or later.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Real-Time PCR Software?

If you are analyzing results from the ViiA 7 Real-Time PCR System, QuantStudio 6 Flex Real-Time PCR System, or QuantStudio 7 Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Real-Time PCR Software following the instructions below:
1. Open the data file in the QuantStudio Real-Time PCR Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.

How can I get a summary of the calibrations performed on the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

You can get a summary of the calibrations from the QuantStudio Software by going to Instrument > Instrument Console > Manage Instrument > Information > Print Calibration Log.

Do I need to back up my calibration files before I upgrade the software for the ViiA7 Real-Time PCR System?

No, you do not need to back up the calibration files as all of the calibration files are stored on the ViiA7 Real-Time PCR instrument. Once the computer software is properly installed and the computer is connected to the instrument, the calibration information will automatically update in the QuantStudio Software.

Can I still start a run on the ViiA7 Real-Time PCR System if the "Calibration is expired" notification is displayed?

Yes, you can still start a run on the ViiA7 Real-Time PCR System if the expired calibration notice displays. This is just a reminder and you can close it to proceed with the run. Additionally, you can change when you want the notifications to show up and how frequently you want to be notified to calibrate the instrument. To do this, go to the toolbar in the QuantStudio Software and select Instrument > Instrument Console. Select your instrument icon and click on Manager Instrument > Maintenance > Calibration Reminders. From the Calibration reminders Settings page you can update when the calibrations expire, turn expiration reminders on/off, and set when the reminders show up.

For Research Use Only. Not for use in diagnostic procedures.