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Description
Recommended positive controls: H1299, HeLaS3, mouse brain. Predicted reactivity: Mouse (80%), Bovine (86%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration. [UniProt].
Specifications
Specifications
| Antigen | LIMCH1 |
| Applications | Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry |
| Classification | Polyclonal |
| Concentration | 1.53 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with 20% glycerol and 0.025% ProClin 300; pH 7 |
| Gene | LIMCH1 |
| Gene Accession No. | Q3UH68, Q9UPQ0 |
| Gene Alias | 3732412D22Rik; Kiaa1102; LIM and calponin homology domains 1; LIM and calponin homology domains-containing protein 1; Limch1; LIMCH1A; LMO7B; mKIAA1102 |
| Gene Symbols | LIMCH1 |
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Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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