Invitrogen™ Lipocalin-2 (LCN2/NGAL) Rat ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ ERLCN2

Catalog No. ERLCN2

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Rat Lipocalin-2 (LCN2/NGAL) ELISA quantitates Rt Lipocalin-2 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Lipocalin-2. Principle of the method The Rat Lipocalin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

Specifications

• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
• For Use With (Equipment): Colorimetric Microplate Reader
• Conjugate: Biotin
• Assay: Solid-phase sandwich ELISA
• Accession Number: P30152
• Calibration Range: 30pg/mL to 8000pg/mL
• Gene ID (Entrez): 170496
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 45 min.
• Research Discipline: Inflammation
• Interassay CV: <12%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: Sip24

• Product Type: ELISA Kit
• Sufficient For: 96 assays
• Protein Family: Proteases, Peptidases & Inhibitors
• Label or Dye: HRP
• Assay Range: 32.77-8000 pg/mL
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Test Time: 1 hr. 20 min.
• Target: Rat
• Synonym: Sip24
• Intraassay CV: <10%
• Target Species: Rat
• Assay Sensitivity: 30 pg/mL