Invitrogen Lipofectamine LTX Reagent with PLUS Reagent
Lipofectamine LTX Reagent with PLUS Reagent
Manufacturer: Invitrogen 15338030
DescriptionLipofectamine LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities. Lipofectamine LTX Reagent is an advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.
Lipofectamine LTX Reagent offers:
Transfect primary cells efficiently
Primary cells are often difficult to transfect. Lipofectamine LTX Reagent, when combined with PLUS Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine LTX Reagent, including primary neural progenitor cells.
Superior delivery into challenging cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine LTX Reagent outperforms competing transfection reagents (see figure). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.
Broad compatibility with cell lines
In addition to being excellent for transfecting primary and challenging cells, Lipofectamine LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.
Lipofectamine LTX strikes the right balance between potency and gentleness, giving you the highest possible percentage of successfully transfected cells. This makes Lipofectamine LTX Reagent the quickest and easiest reagent for establishing stable transfectants from challenging or refractory cell types.
Simple, rapid, serum-compatible protocol
Lipofectamine LTX Reagent offers a streamlined protocolno need to remove transfection complexes or change/add medium following transfection. A simple optimization protocol helps you get the best results quickly, so you'll spend less hands-on time, and get faster results.
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|Plasmid DNA, RNAi Plasmids (shRNA, miR)|
|Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells|
|Each unit contains one vial of Lipofectamine® LTX Reagent (0.3 ml) and one vial of Plus Reagent (250 µl). Store at 2–8°C.
Guaranteed stable for 6 months when properly stored.
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