MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Catalog Number	Mfr. No.	Quantity	Product Type	Sensitivity
WBLUF0500	MilliporeSigma™ WBLUF0500	500 mL	HRP Substrate	∼400 fg
WBLUR0500	MilliporeSigma™ WBLUR0500	500 mL	HRP Substrate	∼1 to 3 pg
WBLUC0500	MilliporeSigma™ WBLUC0500	500 mL	HRP Substrate	∼6 pg

Description

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

• Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

• Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
• Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
• Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

• Immobilon Classico premixed reagent for everyday detection needs
• Immobilon Crescendo premixed reagent for detection in the picogram range
• Immobilon Forte premixed reagent for detection in the femtogram range

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Specifications

• For Use With (Application): Dot/Slot Blot