Invitrogen™ Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), APC, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-245-689	Invitrogen™ 17578282	100 μg

Description

Description: The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity. Applications Reported: This eBio4E5 (4E5) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4E5 (4E5) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can...

Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R.

Specifications

• Antigen: Ly-49D
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60651
• Gene ID (Entrez): 16635
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: eBio4E5 (4E5)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: Klra4
• Gene Alias: Chok; killer cell lectin-like receptor 4; killer cell lectin-like receptor subfamily A member 32; killer cell lectin-like receptor subfamily A member 33; killer cell lectin-like receptor, subfamily A, member 4; Klra32; Klra33; Klra4; Ly49d; Ly-49d; Ly49-d; ly49r>129>; Lymphocyte antigen 49d; T-cell surface glycoprotein Ly-49D
• Gene Symbols: Klra4
• Product Type: Antibody