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Invitrogen™ Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), Biotin, eBioscience™
Rat Monoclonal Antibody
Supplier: Invitrogen™ 13578282

Description
Description: The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity. Applications Reported: This eBio4E5 (4E5) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4E5 (4E5) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can...
Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R.
Specifications
Ly-49D | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q60651 | |
Klra4 | |
Affinity Chromatography | |
RUO | |
16635 | |
4°C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBio4E5 (4E5) | |
Biotin | |
Klra4 | |
Chok; killer cell lectin-like receptor 4; killer cell lectin-like receptor subfamily A member 32; killer cell lectin-like receptor subfamily A member 33; killer cell lectin-like receptor, subfamily A, member 4; Klra32; Klra33; Klra4; Ly49d; Ly-49d; Ly49-d; ly49r>129>; Lymphocyte antigen 49d; T-cell surface glycoprotein Ly-49D | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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