Invitrogen™ Ly-49E/F Monoclonal Antibody (CM4), APC, eBioscience™

Catalog No.	Quantity
50-152-25	25 μg

Catalog No. 50-152-25 Supplier Invitrogen™ Supplier No. 17584880

Description

Rat Monoclonal Antibody

Description: This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F. The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A). Applications Reported: This CM4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CM4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be care...

The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).

Specifications

• Antigen: Ly-49E/F
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: CM4
• Concentration: 0.2 mg/mL
• Conjugate: APC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Klra5
• Gene Accession No.: Q60652, Q60653
• Gene Alias: class I MHC receptor KLRA5; killer cell lectin-like receptor 5; Killer cell lectin-like receptor 6; killer cell lectin-like receptor, subfamily A, member 5; killer cell lectin-like receptor, subfamily A, member 6; Klra5; Klra6; Ly49e; Ly-49e; Ly49-e; Ly49E receptor; Ly49f; ly-49f; Ly49-f; Lymphocyte antigen 49e; lymphocyte antigen 49f; T-cell surface glycoprotein Ly-49E; T-cell surface glycoprotein Ly-49F
• Gene Symbols: Klra5, Klra6
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16636, 16637
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a