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Invitrogen™ Ly-49I Monoclonal Antibody (YLI-90), PE, eBioscience™
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Catalog No. 5010969
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100 μg
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Catalog No. Quantity
50-109-69 100 μg
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Catalog No. 50-109-69 Supplier Invitrogen™ Supplier No. 12589582
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Mouse Monoclonal Antibody

Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice. Applications Reported: YLI-90 has been reported for use in flow cytometric analysis. Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
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Specifications

Antigen Ly-49I
Applications Flow Cytometry
Classification Monoclonal
Clone YLI-90
Concentration 0.2 mg/mL
Conjugate PE
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Klra9
Gene Accession No. 0
Gene Alias 5E6; Killer cell lectin-like receptor 3; killer cell lectin-like receptor subfamily A, member 9; Klra3; Klra9; Ly-49; Ly49C; Ly-49c; Ly49I; Ly49I receptor; Ly-49I receptor; LY49I1; Lymphocyte antigen 49c; Nk2.1; T-cell surface glycoprotein Ly-49C
Gene Symbols Klra9
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 16640
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
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