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  6. Invitrogen™ Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™, Invitrogen™ 

Invitrogen™ Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™, Invitrogen™

Catalog No. 501129110
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50-112-9110 25 μg
50-112-9287 100 μg
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Catalog No. 50-112-9110 Supplier Invitrogen™ Supplier No. 47593280
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Description

Rat Monoclonal Antibody

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells. In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release. Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Ly-6C
Applications Flow Cytometry
Classification Monoclonal
Clone HK1.4
Concentration 0.2 mg/mL
Conjugate APC-eFluor 780
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Ly6c1
Gene Accession No. P0CW02
Gene Alias AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C
Gene Symbols Ly6c1
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 17067
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2c κ
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