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Description
Description: This 49-H4 monoclonal antibody reacts with mouse Ly-6D, which is also known as ThB. This member of the Ly6 family of GPI-anchored cell surface glycoproteins is expressed on thymocytes and splenic B cells. Moreover, common lymphoid progenitor cells that express Ly-6D have been shown to commit to the B cell lineage. Expression of Ly-6D has also been associated with Rag-1 expression along with a reduced ability to differentiate into T or NK cells. Finally, reports indicate that the level of Ly-6D expression varies in different mouse strains. Applications Reported: This 49-H4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 49-H4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
May act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. Marks the earliest stage of B-cell specification. [UniProt].
Specifications
| Ly-6D | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| P35459 | |
| Ly6d | |
| Affinity chromatography | |
| RUO | |
| 17068 | |
| 4°C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| 49-H4 | |
| eFluor 450 | |
| Ly6d | |
| E48; E48 antigen; Ly61; Ly-61; LY6D; Ly-6D; lymphocyte antigen 6 complex, locus D; lymphocyte antigen 6D; Thb; thymocyte B-cell antigen | |
| Rat | |
| 25 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2c κ |
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