Lymphotactin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-271-8490	Invitrogen™ 500P40BT500UG	500 μg

Description

AA Sequence of recombinant protein: GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Lymphotactin (XCL1). Anti-Human Lymphotactin (XCL1)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hLymphotactin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Lymphotactin (500-P40) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLymphotactin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P40BT-1MG will be provided as 2 x 500 μg

Lymphotactin, otherwise known as XCL1, is a member of the C-chemokine family that uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation. Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.

Specifications

• Antigen: Lymphotactin
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P47992
• Gene ID (Entrez): 6375
• Immunogen: E.coli-derived Recombinant Human Lymphotactin (XCL1).
• Purification Method: Antigen affinity chromatography
• Gene: Xcl1

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: AI661682; ATAC; C motif chemokine 1; chemokine (C motif) ligand 1; cytokine SCM-1; Lptn; LTN; Lymphotactin; lymphotaxin; SCM1; SCM-1; SCM1A; SCM-1a; SCM1alpha; SCM-1alpha; SCM-1-alpha; SCM1beta; SCM-1beta; SCM-1-beta; SCYC1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); small-inducible cytokine C1; XC chemokine ligand 1; X-C motif chemokine ligand 1; XCL1
• Gene Symbols: Xcl1
• Primary or Secondary: Primary
• Content And Storage: -20°C