Gibco™ Lysobacter Enzymogenes Arg-C Recombinant Protein, PeproTech®

Catalog No.	Quantity
450-54-20UG	20 μg
450-54-5UG	5 μg

Catalog No. 450-54-20UG Supplier Gibco™ Supplier No. 4505420UG

Description

Recombinant Protein

450-54-100UG will be provided as multiple 5 μg (450-54-5UG) and 20 μg (450-54-20UG) vials. Recombinant Lysobacter Enzymogenes Arg-C is a 26.8 kDa protease consisting of 252 amino acid residues including a C-terminal His-Tag. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at, or adjacent to specific residues, or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes, including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Arg-C specifically cleaves at the carboxyl side of Arginine residues. Arg-C has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl-containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants, and by Co^2+, Cu2+, Cd^2+, and heavy metal ions.

Specifications

• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Molecular Weight (g/mol): 26.8 kDa
• Name: Lysobacter Enzymogenes Arg-C
• Quantity: 20 μg
• Source: Insect cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Common Name: Arg-C
• Biological Activity: The reaction is measured as an increase in absorbance at 253 nm resulting from the hydrolysis of N-benzoyl-L-arginine ethyl ester (BAEE).
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: GVGDIGSSDY CEKDIVCRVK PSAEFLSASK SVARMVFTPK TGYTGYCSGT LLNNSNSPKR QLFWSAAHCI STQKVANTLQ TYWLYDATGC DNDTLSDKAV TLTGGATLLH SHATRDTLLL ELKSAPPSGA YYAGWNSSAI ATKGTAIEGI HHPSGDLKKY SLGSVTALSS TIDGKKPLTK VAWTTGVTEG GSSGSGLFTI SSTSGYQLRG GLYGGTSYCS APSDPDYYSQ LDGVWSSIKT YFSPHHHHHH HH
• Content And Storage: -20°C
• Expression System: Insect cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.