Learn More
Invitrogen™ LyticBLAzer™-FRET B/G Homogeneous Kit
Description
The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ FRET B/G Substrate Kit Advantages:
• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times
LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times
Specifications
Specifications
| Product Line | LyticBLAzer |
| For Use With (Equipment) | Fluorometer, Microplate Reader |
| Content And Storage | LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request. LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request. LyticBLAzer™_h-BODIPY™ FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY™ FL Substrate (provided lyophilized) should be stored at -20C, and protected from light. LyticBLAzer™_h-BODIPY™ FL Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. LyticBLAzer™-BODIPY™ FL Loading Kits include LyticBLAzer™-BODIPY™ FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY™ FL substrate is provided lyophilized and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored (at 22°C to 25°C, protected from direct light.) |
| Detection Method | Fluorescence |
| Product Type | Kits for Analysis of Beta-Lactamase Activity |
| Quantity | 3.75 L |
| Substrate | LyticBLAzer™-FRET B/G |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.