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Thermo Scientific™ LyticBLAzer™-FRET B/G Kit, 10X
Description
The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ FRET B/G Substrate Kit Advantages:
• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times
LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times
Specifications
Specifications
| Product Line | LyticBLAzer |
| For Use With (Equipment) | Fluorometer, Microplate Reader |
| Includes | 10 Kits |
| Content And Storage | LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request. LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request. LyticBLAzer™_h-BODIPY™ FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY™ FL Substrate (provided lyophilized) should be stored at -20C, and protected from light. LyticBLAzer™_h-BODIPY™ FL Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light. LyticBLAzer™-BODIPY™ FL Loading Kits include LyticBLAzer™-BODIPY™ FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY™ FL substrate is provided lyophilized and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored (at 22°C to 25°C, protected from direct light.) |
| Detection Method | Fluorescence |
| Product Type | Kits for Analysis of Beta-Lactamase Activity |
| Quantity | 15 mL |
| Substrate | LyticBLAzer™-FRET B/G |
For Research Use Only. Not for use in diagnostic procedures.
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