Novus Biologicals™ M-CSF Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human M-CSF.

Specifications

• Assay Range: 23.44 pg/mL
• Assay Sensitivity: 23.44-1500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human M-CSF.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: colony stimulating factor 1 (macrophage), CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, M-CSF, MCSFlanimostim, MGC31930
• Gene ID (Entrez): 1435
• Gene Symbol: CSF1
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 22 ng of recombinant Human M-CSF.
  Capture Antibody: 0.5 mg/mL of rabbit anti-Human M-CSF monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human M-CSF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Biologically Active Proteins, Cell Cycle and Replication, Cytokine Research, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: M-CSF
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.