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R&D Systems™ MagCellect Plus Human EGFR+ Cell Isolation Kit

Catalog No. MAGH124
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Description

The MagCellect™ Plus Human EGFR+ Cell Isolation Kit is designed to isolate Epidermal Growth Factor Receptor (EGFR) expressing cells via a positive selection principle. The resulting cell preparation is highly enriched with EGFR+ cells. Typical purity of recovered EGFR+ cells ranges from 80-95%.

A mononuclear cell suspension is first incubated with the Human EGFR Biotinylated Antibody that targets the desired cells. MagCellect™ Streptavidin (SA) Ferrofluid is added to the reaction that allows the SA coated nanoparticles to interact with the cells tagged with the monoclonal antibodies. The tube containing the cell suspension is then placed within a magnetic field. Magnetically tagged cells will migrate toward the magnet (desired cell fraction), leaving the untagged cells (unwanted cell population) in suspension to be harvested by aspiration while the tube remains in the magnetic field. The tube containing the magnetically selected (desired) cells is then removed from the magnet and the cells are resuspended in MagCellect™ Plus Buffer or tissue culture media. The enriched cell preparation is then available for a variety of applications including tissue culture, immune status monitoring and flow cytometry.

Kit Contents:

  • Human EGFR Biotinylated Antibody(625 μL of Human EGFR biotinylated antibody in a phosphate buffered solution containing BSA and preservative)
  • Human EGFR Alexa Fluor™ 647 Antibody (125 μL of Alexa Fluor™ 647-conjugated Goat anti-human EGFR Detection antibody)
  • Streptavidin Ferrofluid (1.25 mL of a solution containing BSA and preservatives)
  • 2 bottles (50 mL/bottle) of a 10X concentrated buffer

Specifications

Synonym avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID (Entrez) 1956
Content And Storage Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Quantity 1 Kit
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