Invitrogen™ MagicMedia™ E. coli Expression Medium, Powder

Catalog No.	Quantity
K6810	1 L
K6815	5 x 1 L

Catalog No. K6810 Supplier Invitrogen™ Supplier No. K6810

Description

Includes

Contains a proprietary Component A (in foil pouch powder format), and Component B (in buffer liquid format).

MagicMedia E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture OD (optical density) or adding inducer. The proprietary MagicMedia formulation enables E. coli growth to reach culture densities 3- to 10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
• Ideal for simultaneous expression of multiple clones for high-throughput applications

MagicMedia E. coli Expression Medium, powder, consists of a proprietary Component A (in foil pouch powder format) and Component B (in buffer liquid format).

MagicMedia E. coli Expression Medium is also available in a ready-to-use, pre-sterilized liquid format (Cat. No. K6803).

Order Info

Store Component A (foil pouches) and Component B buffer (liquid) at Room Temperature (15 - 30°C).

Specifications

• Content And Storage: Store Component A (foil pouches) and Component B (liquid) at room temperature (15–30°C).
• Form: Powder, Liquid
• Format: Kit
• Product Type: Microbiology Media
• Product Line: MagicMedia
• Quantity: 1 L
• Species: Escherichia coli

Frequently Asked Questions (FAQs)

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.