MilliporeSigma™ Magna Nuclear RIP™ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit

Catalog No.	Quantity
17-105-22	24 Assays

Catalog No. 17-105-22 Supplier MilliporeSigma™ Supplier No. 1710522

Description

Includes

• Nuclei Isolation Buffer
• RIP Lysis Buffer
• Protein A/G Magnetic Beads
• RIP Lysis Buffer
• 0.5M EDTA
• DNase I (RNase Free) 2U/μL
• DNase I Reaction Buffer
• Protease Inhibitor Cocktail III, Animal Free
• RNAse Inhibitor

Requires

• Antibody of interest for RNA-binding protein immunoprecipitation (RIP)
• Negative Control Antibody
• Precipitation Carrier (e.g. Pellet Paint™ Co-Precipitant or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation
• Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction (optional)

Specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs

• Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
• Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 100,000 cells (native)
• Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
• Suitable for analysis by RT-qPCR or RIP-seq
• Complete set of reagents and detailed protocol to enable first-time success

Specifications

• Content And Storage: Store components at the temperatures indicated on the labels
• For Use With (Application): RNA Binding Protein Immunoprecipitation (RIP), Protein Interaction Assays
• Quantity: 24 Assays
• Stability: Kit components are stable for 6 months from date of shipment when stored as directed