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Invitrogen™ Multispecies MAPK Family Activation InstantOne™ ELISA Kit

Catalog No. 858619511
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Quantity:
96 Tests

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), p38 MAPK (human, mouse), and JNK 1/2 (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPKBP-1 (mitogen-activated protein kinase binding protein 1), also known as JNKBP-1, is a 1,514 amino acid protein that contains twelve WD repeats. Induced by TRAF2 (TNF receptor-associated factor 2) and Tak1 (TGF-β-activated kinase 1), MAPKBP-1 is thought to act an adaptor protein for NFκB (nuclear factor κ-B) activation. MAPKBP-1 interacts with JNK3 and may promote TRAF2 polyubiquitination. MAPKBP-1 exists as six alternatively spliced variants and is encoded by a gene located on human chromosome 15. Human chromosome 15 houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P27361,P28482,P45983,Q16539
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Human, Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1432, 5594, 5595
Gene Symbol ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK,JNK,JNK-46,JNK1,JNK1A2,JNK21B1/2,PRKM8,SAPK1,SAPK1c,CSBP,CSBP1,CSBP2,CSPB1
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym ERK, p38, JNK
Target JNK1/JNK2/JNK3/ERK1/2/p38 MAPK alpha
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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