Invitrogen™ Marginal Zone B Cells Monoclonal Antibody (HIS57), eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 14948982

Catalog No. 50-112-9349

Description

Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low. Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: This HIS57 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.

Specifications

• Antigen: Marginal Zone B Cells
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunocytochemistry
• Clone: HIS57
• Conjugate: Unconjugated
• Gene Alias: MZ-B; MZB1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG1 κ