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Marginal Zone B Cells Mouse Anti-Rat, Brilliant Violet 421, Clone: HIS57, BD Optibuild™

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BDB743341 50 μg
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Description

Mouse Monoclonal Antibody

The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen. In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations. Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM. The HIS57 mAb does not stain granulocytes and thymocytes. Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles. This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Marginal Zone B Cells
Classification Monoclonal
Clone HIS57
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen Rat Splenic B Cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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