Marginal Zone B Cells Mouse Anti-Rat, Brilliant Violet 711, Clone: HIS57, BD Optibuild™

Catalog No.	Quantity
BDB743345	50 μg

Catalog No. BDB743345 Supplier BD Biosciences Supplier No. 743345

Description

Mouse Monoclonal Antibody

The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen. In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations. Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM. The HIS57 mAb does not stain granulocytes and thymocytes. Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles. This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: Marginal Zone B Cells
• Classification: Monoclonal
• Clone: HIS57
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 711
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Rat Splenic B Cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ