Thermo Scientific™ Maxima Reverse Transcriptase (200 U/µL)
Increase synthesis rates with Thermo ScientificTM MaximaTM Reverse Transciptase, an advanced enzyme developed through in vitro evolution of M-MuLV RT.
Supplier: Thermo Scientific™ EP0743
Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as Rnase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.
Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 μg) at elevated temperatures (50 to 65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).
Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb (see supporting data). The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 min.
- High yields of full-length cDNA up to 20 kb
- Active up to 65°C
- Thermostable -90% active after incubation at 50°C for 60 minutes
- High sensitivity -reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 μg)
- Efficient -complete cDNA synthesis in 15 to 30 minutes
- Incorporates modified nucleotides
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
• Maxima Reverse Transcriptase, 4 x 10,000 units (200 U/μL)
|Up to 20 kb|
|50°C to 55°C|
|4 x 10,000 Units|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok