Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.

$72.65 - $737.65

Specifications

Concentration 200 U/μL
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
FEREP0741
View Documents
Thermo Scientific™
EP0741
2,000 Units
Each for $72.65

Save 20%

Reg : $91.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
FEREP0742
View Documents
Thermo Scientific™
EP0742
10,000 Units
Each for $229.65

Save 20%

Reg : $286.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
FEREP0743
View Documents
Thermo Scientific™
EP0743
4 x 10,000 Units
Each for $737.65

Save 20%

Reg : $924.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
Description

Description

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.

Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 μg) at elevated temperatures (50–65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).

Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 minutes

Features of Maxima Reverse Transcriptase include:
• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity allows reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 μg)
• Efficient formula for complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides

Applications
• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension

Also available
• Maxima Reverse Transcriptase (Cat. No. EP0741)
• Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Cat. No. K1641)
• Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase (Cat. No. K1671)

Specifications

Specifications

200 U/μL
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit