Invitrogen™ Human MBL (MBL2) ELISA Kit

Description

ELISA

The Human MBL(MBL2) ELISA quantitates Hu MBL in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MBL. Principle of the method The Human MBL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.

Specifications

• Accession Number: P11226
• Assay Range: 0.034 to 25 ng/mL
• Assay Sensitivity: 0.03 ng/mL
• Calibration Range: 0.03 ng/mL to 25 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA antibody pair detects human MBL. Other species not determined yet.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4153
• Gene Symbol: COLEC1,HSMBPC,MBLD,MBP,MBP-C,MBP1,MBPD,MBL2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Microbiology
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: RP11-94H3.1, COLEC1, HSMBPC, MBLD, MBP, MBP-C, MBP1, MBPD, MBL2
• Target: MBL2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins