Cytiva MBPTrap™ HP Prepacked Columns

Description

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

• Prepacked with Dextrin Sepharose tagged with maltose binding protein (MBP).
• Binding capacity is fusion protein dependent but can be as much as 16 mg/ml media
• Maximum pressure: 3 bar
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
• 1 and 5mL polypropylene columns can be used either manually using a syringe or on an instrument

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: >7

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

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Specifications

• Buffer: Aqueous Buffer
• Product Type: Dextrin Sepharose HP prepacked column
• Bed Volume: 1mL
• Diameter (English) Inner: 0.62 in.
• Diameter (Metric) Inner: 16mm
• Quantity: 5 x 5mL
• Height (English): 0.98 in.
• Height (Metric): 25mm
• Flow Rate: <20 mL/min.
• Material: Polypropylene
• Ligand Type: Dextrin
• Max. Pressure: 3 bar
• pH Range: >7
• Particle Size: 34μm
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 2° to 8°C
• Column Type: Ion Exchange Column
• For Use With (Application): Purification of MBP tagged Proteins