Novus Biologicals™ MCAM/CD146 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human MCAM/CD146.

Specifications

• Assay Range: 31.25 pg/mL
• Assay Sensitivity: 11-700 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human MCAM/CD146.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD146, CD146 antigen, cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18Gicerin
• Gene ID (Entrez): 4162
• Gene Symbol: MCAM
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human MCAM/CD146 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of mouse anti-Human MCAM/CD146 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 80 ng of recombinant Human MCAM/CD146.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Mesenchymal Stem Cell Markers, Phospho Specific, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: MCAM/CD146
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 700 pg/mL is recommended.