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Novus Biologicals™ MCAM/CD146 Antibody Pair [HRP]

Monoclonal Antibody Pair

Manufacturer:  Novus Biologicals™ NBP2794455PLATES

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Catalog No. NB009930

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This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human MCAM/CD146.


Antibody Pairs
31.25 pg/mL
Cancer, Mesenchymal Stem Cell Markers, Phospho Specific, Stem Cell Markers
1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 700 pg/mL is recommended.
Sandwich ELISA
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human MCAM/CD146.
Sandwich ELISA
  • Detection Antibody: 0.2 mg/mL of rabbit anti-Human MCAM/CD146 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Capture Antibody: 0.5 mg/mL of mouse anti-Human MCAM/CD146 monoclonal antibody (in PBS, pH 7.4).
  • Standard: Each vial contains 80 ng of recombinant Human MCAM/CD146.
  • Storage is content dependent.
    5 Plates
    11-700 pg/mL
    CD146, CD146 antigen, cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18Gicerin
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