Invitrogen™ MCL-1 Monoclonal Antibody (LVUBKM), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-4615	100 Tests

Catalog No. 50-112-4615 Supplier Invitrogen™ Supplier No. 53904742

Description

Mouse Monoclonal Antibody

Description: This LVUBKM monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. Although it is expressed in both immune and non-immune cells, highest levels of Mcl-1 expression are seen in hematopoietic lineage cells. PI3K activation of AKT results in destabilization and degradation of GSK3 beta, which prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintece of mature lymphocytes. Applications Reported: This LVUBKM antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This LVUBKM antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.

Specifications

• Antigen: MCL-1
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: LVUBKM
• Concentration: 5 μL/Test
• Conjugate: Alexa Fluor 488
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: MCL1
• Gene Accession No.: P97287, Q07820
• Gene Alias: AW556805; BCL2 family apoptosis regulator; BCL2L3; bcl2-L-3; Bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; EAT; Induced myeloid leukemia cell differentiation protein Mcl-1; induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MCL-1S; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1; myeloid cell leukemia sequence 1 (BCL2-related); TM
• Gene Symbols: MCL1
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17210, 4170
• Target Species: Human, Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

For Research Use Only.