Invitrogen™ MCP-4 Monoclonal Antibody, PeproTech®

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 500M70500UG

Catalog No. 50-0M7-0500UG

Description

AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT. Preparation: Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography. Sandwich ELISA: Assuming 100 μL/well, a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.

Specifications

• Antigen: MCP-4
• Classification: Monoclonal
• Formulation: PBS with no preservative
• Gene Accession No.: Q99616
• Gene Symbols: CCL13
• Immunogen: E.coli-derived Recombinant Human MCP-4 (CCL13).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Gene: CCL13
• Gene Alias: C-C motif chemokine 13; C-C motif chemokine 13, long chain; C-C motif chemokine 13, medium chain; C-C motif chemokine 13, short chain; C-C motif chemokine ligand 13; CCL 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; CK-beta-10; H-MCP-4; MCP4; MCP-4; Monocyte chemoattractant protein 4; monocyte chemotactic protein 4; NCC1; NCC-1; new CC chemokine 1; SCYA13; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13; small-inducible cytokine A13
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 6357
• Content And Storage: -20°C
• Form: Lyophilized