MilliporeSigma™ MDR1 Efflux Assay

Description

Includes

• Sterile 5x RPMI-1640 - One bottle containing 200ml of sterile 5x concentrated RPMI-1640
• Sterile 30% BSA - One bottle containing 35ml sterile-filtered 30% bovine serum albumin (BSA) in PBS
• Gentamicin Solution, 1000x - One vial containing 1ml sterile-filtered 50mg/ml gentamicin
• DiOC2(3) Solution - One vial containing 50μl of DiOC2(3) (3,3'-diethyloxacarbocyanine iodide) at 1 mg/ml in DMSO
• Rhodamine 123 Solution - One vial containing 0.5ml of rhodamine 123 at 1mg/ml in DMSO
• Vinblastine Solution - One vial containing 150μl of 22mM vinblastine in DMSO
• DMSO - One vial containing 150μl DMSO
• Propidium Iodide Stock Solution - One vial containing 1ml of 50μg/ml propidium iodide in PBS

Includes two of the best characterized & most commonly used multidrug resistance ABC transporter substrates, DiOC2(3) and rhodamine 123

The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under physiologic conditions by directly measuring the relative fluorescence of cell populations that actively extrude fluorescent multidrug resistance transport substrates

Specifications

• Content And Storage: Stored at 2°C to 8°C upon arrival
• Detection Method: Fluorescence
• For Use With (Application): DiOC2(3) and rhodamine 123
• Quantity: 100 Assays
• Stability: Stable for 1 year after receipt