MilliporeSigma™ MED28, Mouse, Unlabeled, Clone: 7E1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC1143

Catalog No. MABC1143MI

Description

Mediator of RNA polymerase II transcription subunit 28 (UniProt Q9H204; also known as Endothelial-derived protein 1, Magicin, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1) is encoded by the MED28 (also known as EG1, FKSG20) gene (Gene ID 80306) in human. MED28 (Magicin) is a subunit of the Mediator complex that is composed of at least 30 subunits and plays an essential role in transcriptional regulation by bridging RNA polymerase II transcriptional machinery and DNA-bound regulatory factors. MED28 is reported to mediate cell surface receptor-mediated signaling and associate with Src-family kinases (e.g. Fyn, Src and Lck), as well as with the Neurofibromatosis 2 (NF2) tumor suppressor merlin. MED28 is associated with both the cytoskeleton and the nucleus, indicating a role as an adaptor/scaffolding protein to relay cellular signals to the cytoskeleton and the nucleus. Homozygous Med28-deletion causes peri-implantation lethality with a loss of pluripotency within the inner cell mass (ICM) of the blastocyst. In addition, Med28-/- embryonic fibroblasts (MEFs) lose the capability to reprogram and Med28 overexpression augments reprogramming efficiency. Upregulated MED28 expression is associated with poor outcome in breast cancer patients, and targeted MED28 downregulation decreases breast cancer cell proliferation and tumor growth.

Specifications

• Antigen: MED28
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q9H204
• Immunogen: GST-tagged recombinant human MED28 lacking N-terminal 12 amino acids.
• Quantity: 100 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_079481
• Target Species: Human
• Form: Purified

• Applications: Immunocytochemistry, Western Blot
• Clone: 7E1
• Conjugate: Unconjugated
• Gene: MED28, EG1, FKSG20
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 7E1 detected MED28 in wild-type, but not Med28-/- murine cells. Clone 7E1 detected Cre expression-induced MED28 knockdown in MEFs with floxed Med28 alleles (Li, L., et al. (2015). PLoS One. 10(10):e0140192).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.