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Novex™ Membrane Blocking Solution

Catalog No. 000105
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Description

Membrane Blocking Solution

Membrane Blocking Solution can be used to block nitrocellulose and PVDF membranes, as well as to dilute antibodies or enzyme conjugates. This solution is a Tris buffer containing bovine serum albumin, goat IgG, Tween 20, and a mixture of other components that can reduce non-specific binding. The Proclin used in this reagent does not inhibit peroxidase or alkaline phosphatase activity. Note that this blocking solution contains normal goat IgG and should not be used in assays where anti-goat immunoglobulins are employed.
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Specifications

Chemical Name or Material Membrane Blocking Solution
Quantity 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

For Research Use Only. Not for use in diagnostic procedures.

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