Molecular Probes™ Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

Description

Vybrant™ Apoptosis Assay Kit #13 *PO-PRO™-1/7-aminoactinomycin D* *for flow cytometry* *200 assays*

This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Quantity: 1 kit
• Format: Tube, Slide
• Product Type: Dead Cell Apoptosis Kit
• Content And Storage: Contains 1 vial of PO-PRO™-1 dye (500 μL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 μL).
  
  Store in refrigerator (2–8°C) and protect from light.
• Flow Cytometer Laser Lines: 405, 488
• Excitation/Emission: PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647
• No. of Reactions: 200
• Shipping Condition: Room Temperature
• Product Line: Vybrant™
• For Use With (Application): Flow Cytometry
• Conjugate: 7-AAD, PO-PRO-1
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer

For Research Use Only. Not for use in diagnostic procedures.