For accurate, sensitive, on-column methylation and detection by GC analysis use Thermo Scientific MethElute Reagent (TMPAH). It is ideally suited for the quantitative methylation and detection of barbiturates, sedatives, xanthine bases, phenolic alkaloids, and phenytoin, giving a single quantitative peak for each substance. When the reagent is heated with drug-containing extracts, serum, or urine the drugs containing reactive amino, hydroxyl, and carboxyl functions will be methylated at the reactive sites.Performance Characteristics
Accuracy comparable or better than the UV/TLC method (GC is superior to UV/TLC when phenobarbitol and phenytoin present)
High precision, with coefficient of variation of 5% or less
High sensitivity; barbiturates deteced down to 0.2 mg/dL
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|Trimethylanilinium hydroxide in Methanol|
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