Methyl-STAT5 (Arg31) Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-2052	Rockland Immunochemicals 600401GH5	100 μg
50-199-2053	Rockland Immunochemicals 600401GH5S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for STAT5 R31/Me2a reacts with human STAT5. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Specifications

• Antigen: Methyl-STAT5 (Arg31)
• Applications: Dot Blot, ELISA, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P42229
• Gene ID (Entrez): 6776
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 0.75 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 30% glycerol and 0.05% sodium azide; pH 7.2
• Gene Alias: AA959963; BOS_19069; Mammary gland factor; mammary gland factor STAT5A; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT; STAT5; Stat5a; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5
• Gene Symbols: STAT5A
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: STAT5A