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  6. Invitrogen™ MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), APC, eBioscience™, Invitrogen™ 

Invitrogen™ MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), APC, eBioscience™, Invitrogen™

Catalog No. p-7090211
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50-112-3175 25 μg
50-112-8675 100 μg
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Catalog No. 50-112-3175 Supplier Invitrogen™ Supplier No. 17599980
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Description

Mouse Monoclonal Antibody

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
TRUSTED_SUSTAINABILITY

Specifications

Antigen MHC Class I (H-2Db)
Applications Flow Cytometry
Classification Monoclonal
Clone 28-14-8
Concentration 0.2 mg/mL
Conjugate APC
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene H2-D1
Gene Accession No. P01895, P01896, P01897, P01899, P01900, P14426, P14427
Gene Alias H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
Gene Symbols H2-D1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 14964
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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For Research Use Only.

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