MHC Class I (H-2Db) Mouse anti-Mouse, eFluor® 450, Clone: 28-14-8, eBioscience™ 

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-4191	Invitrogen 48599980	25 μg

Description

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

Specifications

• Antigen: MHC Class I (H-2Db)
• Target Species: Mouse
• Isotype: IgG2a, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!

• Clone: 28-14-8
• Conjugate: eFluor™ 450
• Applications: Flow Cytometry
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.