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MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eFluor™ 450, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
$316.00
Specifications
Antigen | MHC Class I (H-2Db) |
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Clone | 28-14-8 |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
48-599-982
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Invitrogen™
48599982 |
100 μg |
Each of 1 for $316.00
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Description
Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.Specifications
MHC Class I (H-2Db) | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
14964 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
28-14-8 | |
Flow Cytometry | |
eFluor 450 | |
Mouse | |
Mouse | |
H2-D1 | |
Primary | |
4°C, store in dark, DO NOT FREEZE! | |
H2-D1 |
For Research Use Only.
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