MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-121-24	Invitrogen™ 13599882	100 μg
50-121-25	Invitrogen™ 13599885	500 μg

Description

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p. Applications Reported: 34-1-2S has been reported for use in flow cytometric analysis. Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class I (H-2Kd/H-2Dd)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 14964, 14972
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: 34-1-2S
• Applications: Flow Cytometry
• Conjugate: Biotin
• Host Species: Mouse
• Target Species: Mouse
• Gene Symbols: H2-D1, H2-K1
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

For Research Use Only.