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MHC Class I (H-2Kd) Mouse anti-Mouse, PerCP-eFluor™ 710, Clone: SF1-1.1.1, eBioscience™ 
Mouse Monoclonal Antibody
$107.00 - $269.00
Specifications
| Antigen | MHC Class I (H-2Kd) |
|---|---|
| Clone | SF1-1.1.1 |
| Target Species | Mouse |
| Conjugate | PerCP-eFluor™ 710 |
| Isotype | IgG2a, kappa |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|
50-245-929
|
Invitrogen
46595780 |
25 μg |
Each for $107.00
|
|
50-245-930
|
Invitrogen
46595782 |
100 μg |
Each for $269.00
|
|
Description
Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed. Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.Specifications
| MHC Class I (H-2Kd) | |
| Mouse | |
| IgG2a, kappa | |
| Monoclonal | |
| Liquid | |
| Antibody | |
| 4° C, store in dark, DO NOT FREEZE! |
| SF1-1.1.1 | |
| PerCP-eFluor™ 710 | |
| Flow Cytometry | |
| Primary | |
| Monoclonal | |
| PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
| RUO |
For Research Use Only.
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