Invitrogen™ MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), FITC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 11598082

Catalog No. 50-993-8

Description

Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis. Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class II (I-Ek)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P04230, P14439
• Gene Symbols: H2-Ea-ps, H2-Eb1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100504404, 14969
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 14-4-4S
• Conjugate: FITC
• Gene: H2-Eb1
• Gene Alias: AI323765; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-IE-alpha; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia3; Ia-3; Ia4; Ia-4; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; major histocompatibility complex H-2E beta chain; MGC117888; MHC class II antigen E alpha; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov