Invitrogen™ Human MICB ELISA Kit

Catalog No.	Quantity
BMS2303	96 Tests

Catalog No. BMS2303 Supplier Invitrogen™ Supplier No. BMS2303

Description

ELISA

The Human MICB ELISA quantitates Hu MICB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MICB.

Principle of the method

The Human MICB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MICB encodes the highly polymorphic MHC (HLA) class I chain-related gene B. The protein product is expressed on the cell surface. It is thought that MICB functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. MICB is broadly recognized by NK cells and T cells with NKG2D receptor on their surface. The complex NKG2D-MICB results in MICB expressing cytolytic T cells and NK cells against epithelial tumor cells.

Specifications

• Accession Number: Q29980
• Assay Range: 3 to 200 pg/mL
• Assay Sensitivity: 1.24 pg/ml
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4277
• Gene Symbol: MICB
• Interassay CV: 7.1%
• Intraassay CV: 4.6
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: MICB
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs