Invitrogen™ Mini Gel Tank and Blot Module Set

Catalog No.	Quantity
NW2000	1 set

Catalog No. NW2000 Supplier Invitrogen™ Supplier No. NW2000

Description

Includes

Mini Gel Tank (Runner Tank; Cassette Clamps, left and right; Tank Base; Tank Lid; and Power Supply Adapters) and two Mini Blot Modules (each with Mini Blot Module with Gasket, Four Blotting Sponges, Blotting Tweezer, Blotting Roller)

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

This set contains a Mini Gel Tank for protein separation and two Mini Blot Modules for Western transfer.

Mini Gel Tank Features

• Compatible with a variety of Novex™ gels, NuPAGE™ gels, and Bolt Bis-Tris Plus gels
• Accommodates up to two gels per run
• Tank design enables side-by-side gel loading and enhanced viewing during use
• Optimized conditions using constant voltage allow Bolt Bis-Tris Plus gels to be run in approximately 35 min.
• Run times vary depending on buffer conditions and power supply being used
• Also accommodates one blot module per chamber, or two blot modules total with side-by-side layout, for convenient Western transfers

Mini Blot Module Features

• Wet transfer device used exclusively with the Mini Gel Tank
• Compatible with Bolt Bis-Tris Plus Gels, accomodating one gel per transfer
• Universal connection and molded gasket make the blot module easy to use
• Inner core of blot module allows for use of less methanol-based transfer buffer per transfer
• At recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 min.

The current model Mini Gel Tank includes cassette clamps with gray cam handles designed for use with the 10cm Bolt minigels as well as NuPAGE and Novex Tris-Glycine minigels.

Specifications

• Content And Storage: • 1 mini gel tank
  • 2 blot modules
  
  
• Capacity: 2 Gels/Run
• Gel Compatibility: Novex™ Mini Gels
• Gel Size: Mini
• Product Line: Invitrogen
• Running Time: 35 min.
• Type: Mini Gel Tank and Blot Module Set
• Quantity: 1 set
• Shipping Condition: Room Temperature
• Max. Voltage: 500 VDC

Frequently Asked Questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Can I use the PowerEase Touch 120W Power Supply for transferring gels with the SureLock Tandem Midi Blot Module, Mini Gel Blot Module, or Novex Semi-Dry Blotter?

No, most transfer applications require high current and therefore, we recommend using a high current power supply like PowerEase Touch 350W Power Supply.

Can I run Bio-Rad gels in the Mini Gel Tank?

No. Bio-Rad gels are of a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Mini Gel Tank.

Which mini gels can I run in the Mini Gel Tank?

All Novex, NuPAGE, and Bolt mini gels can be run in the Mini Gel Tank, including:
- NuPAGE Tris-Acetate Protein Gels
- 10 cm Bolt Bis-Tris Plus Gels
- NuPAGE Bis-Tris Protein Gels
- Novex Tricine Protein Gels

Note: Refer to the user manual for specific run conditions for each gel type.

What comes with the Mini Gel Tank?

The Mini Gel Tank includes the Runner Tank, Cassette Clamps (left & right), Tank Base, Tank Lid, and Power Supply Adapters.

I am using the Mini Gel Tank and am not able to see the sample wells for loading the sample. Can you please suggest some tips?

We recommend marking the cassette at the bottom of the wells with a marker pen prior to placing the cassette in the electrophoresis tank.

I have set up my gel to run in the Mini Gel Tank and have switched on the power supply, but my gel is not running and the current reading on the power supply is very low (close to zero). What is the problem?

Here are possible causes and solutions:

- Tape left on the bottom of the cassette. Remove tape from bottom of cassette.
- Connection to power supply not complete. Check all connections with a voltmeter for conductance.
- Insufficient buffer level. Make sure there is sufficient buffer in the electrophoresis tank to cover the wells of the gel.

My gel run in the Mini Gel Tank is faster than normal with poor resolution. What is the problem?

Here are possible causes and solutions:

- Buffers are too concentrated or incorrect. Check buffer recipe; dilute or re-make if necessary.
- Current is set at a higher limit. Decrease current to recommended running conditions (see Page 8 of the manual).

My Mini Gel Tank gel run is taking longer than usual. What is the problem?

Here are possible causes and solutions:

- Buffers are too dilute. Check buffer recipe; remake if necessary.
- Buffer chamber is leaking. Make sure the cassette clamp is firmly seated, the gaskets are in place and the cassette clamp is locked.
- Current is set too low. Set correct current.

Can I purchase replacement parts for the Mini Gel Tank?

Here are the replacement parts we offer for the Mini Gel Tank:
Replacement part - Cat. No.
Gel Runner Tank - B4478641
Mini Gel Tank Lid - A25944
Mini Gel Tank Base - A25950
Cassette Clamp, left - A25946
Cassette Clamp, right - A25945
Cassette Clamp Cam Handle Set - A26732

How do you suggest cleaning the Mini Gel Tank?

Here are our recommendations:

- When electrophoresis is complete, dispose of the buffer appropriately. Rinse the tank with water to remove residual buffer.
- Clean the surface of the Mini Gel Tank with a soft non-abrasive, lint-free cloth dampened with water.
- Do not use harsh detergents or solvents to clean the unit.

What is the tolerance of the Mini Gel Tank to organic solvents?

The Mini Gel Tank is not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.

Can I use the XCell SureLock Mini Cell or Mini Gel Tank with the Precise Tris-HEPES gels?

The Precise Tris-HEPES gels are compatible with the XCell SureLock Mini Cell or Mini Gel Tank when used with adaptor plates. Note: Two adaptor plates are required when running just one gel and one adaptor plate is required when running two gels using the XCell SureLock Mini Cell or Mini Gel Tank.

Which gel chamber should I use to run your Invitrogen Mini and Midi protein gels?

Our Original Bolt Bis-Tris Plus Mini gels (Cat. No. BGxxxxxBOX, discontinued as of December 31, 2014) can only be run in the Bolt Mini Gel Tank (discontinued as of December 31, 2014, and will be offered until inventory is depleted).

Our New Bolt Bis-Tris Plus Mini gels (Cat. No. NWxxxxxBOX), as well as our Invitrogen Mini gels and NuPAGE Mini gels can be run using the Mini Gel Tank, or XCell SureLock Mini-Cell. To run these gels using the Bolt Mini Gel Tank (discontinued as of December 31, 2014), upgrading of the tank is necessary by replacing the black 10.5 cm cassette clamp cam handles with gray 10 cm cassette clamp cam handles (Cat. No. A26732, Cassette Clamp Cam Handle Set). Instructions for replacement of the cam handles can be found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/mini-gel-tank/resources-upgrading-bolt-mini-gel-tank.html).

Our Midi gels can be run using the XCell4 SureLock Midi-Cell.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.