Invitrogen™ Mouse MIP-3 alpha (CCL20) ELISA Kit

Catalog No.	Quantity
EMCCL20X5	5 x 96 Tests

Catalog No. EMCCL20X5 Supplier Invitrogen™ Supplier No. EMCCL20X5

Description

ELISA

The Mouse Macrophage Inflammatory Protein 3 alpha (CCL20) (Ms MIP-3α) ELISA quantitates Ms MIP-3α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-3α. Principle of the method The Mouse MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

Specifications

• Accession Number: beta chemokine exodus-1; beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; C-C motif chemokine 20; C-C motif chemokine ligand 20; CCL20; CCL20(1-64); CCL20(1-67); CCL20(2-70); chemokine (C-C motif) ligand 20; CKb4; Exodus; exodus-1; LARC; Liver and activation-regulated chemokine; macrophage inflammatory protein 3 alpha; MIP 3 A; MIP 3 A; MIP 3 alpha; MIP 3 A; MIP3 a; MIP3 alpha; MIP3 a; MIP-3 [a]; MIP3A; MIP-3 A; MIP-3-alpha; M-MIP-3-alpha; SCYA20; small inducible cytokine subfamily A (Cys-Cys), member 20; small inducible cytokine subfamily A20; small-inducible cytokine A20; St38
• Assay Range: 2.06 to 1500 pg/mL
• Assay Sensitivity: 3 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20297
• Gene Symbol: CCL20
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: CCL20
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins