Molecular Probes™ Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Description

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Quantity: 1 kit
• Format: Tube, Slide
• Product Type: Mitochondrial Membrane Apoptosis Kit
• Content And Storage: Contains 1 vial of annexin, Alexa Fluor™ 488 conjugate (250 μL), 3 vials of MitoTracker™ red (50 μg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 μL).
  
  Store in refrigerator (2–8°C) and protect from light.
• Flow Cytometer Laser Lines: 488
• Excitation/Emission: Mitotracker™ red 579⁄599, Alexa Fluor™ 488: 499⁄521
• No. of Reactions: 50
• Shipping Condition: Wet Ice
• Product Line: Alexa Fluor™, MitoTracker™
• For Use With (Application): Flow Cytometry
• Conjugate: Alexa Fluor 488, MitoTracker Red
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.