Mitochondrial Pyruvate Carrier 2 (BRP44), Mouse anti-Human, Mouse, Clone: , Millipore Sigma™

Catalog No.	Quantity
MABS191425	25 μg

Catalog No. MABS191425 Supplier MilliporeSigma Supplier No. MABS191425UG

Description

Mouse Monoclonal Antibody

Anti-Mitochondrial Pyruvate Carrier 2 (BRP44), Cat. No. MABS1914, is a mouse monoclonal antibody that detects human and mouse Mitochondrial pyruvate carrier 2 and has been tested for use in Immunoprecipitation and Western Blotting.

Mitochondrial pyruvate carrier 2 (UniProt: O95563; also known as Brain protein 44, MPC2) is encoded by the MPC2 (also known as BRP44) in human. MPC2 is a multi-pass inner mitochondrial membrane protein that mediates the imports pyruvate into mitochondria. Pyruvate is an essential component in intermediary metabolism that occupies a key crossroads position between cytosolic glycolysis and mitochondrial metabolism. MPC2 contains 3 helical domains (aa 41-61; 73-90; and 96-115). In mammalian cells mitochondrial pyruvate carrier is composed on two paralogous subunits known as MPC1 and MPC2 that heteromerize to form a functional complex. It has been reported that MPC2 can also homomerize, however only MPC1/MPC2 heteromeric forms are functional. Complete absence of MPC2 is shown to cause embryonic lethality in murine models, however, murine cell lines that express an N-terminal truncated MPC2 protein (Mpc2 16) are reported to be viable, but exhibit significantly reduced capacity for mitochondrial pyruvate oxidation. (Ref.: Compan, V., et al. (2015). Mol. Cell 59(3); 491-501; Vigueira, PA., et al. (2014). Cell Rep. 7(6); 2042-2053).

Specifications

• Antigen: Mitochondrial Pyruvate Carrier 2 (BRP44)
• Applications: Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates. Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland).. Western Blotting Analysis: 4 μg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate. Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Mitochondrial pyruvate carrier 2;Brain protein 44;BRP44
• Gene Symbols: MCP2;BRP44
• Host Species: Mouse
• Immunogen: A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.
• Target Species: Human, Mouse
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1