Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Mitochondrial Pyruvate Carrier 2 (BRP44), Mouse anti-Human, Mouse, Clone: , Millipore Sigma™
Mouse Monoclonal Antibody
Supplier: MilliporeSigma MABS191425UG
Description
Anti-Mitochondrial Pyruvate Carrier 2 (BRP44), Cat. No. MABS1914, is a mouse monoclonal antibody that detects human and mouse Mitochondrial pyruvate carrier 2 and has been tested for use in Immunoprecipitation and Western Blotting.
Mitochondrial pyruvate carrier 2 (UniProt: O95563; also known as Brain protein 44, MPC2) is encoded by the MPC2 (also known as BRP44) in human. MPC2 is a multi-pass inner mitochondrial membrane protein that mediates the imports pyruvate into mitochondria. Pyruvate is an essential component in intermediary metabolism that occupies a key crossroads position between cytosolic glycolysis and mitochondrial metabolism. MPC2 contains 3 helical domains (aa 41-61; 73-90; and 96-115). In mammalian cells mitochondrial pyruvate carrier is composed on two paralogous subunits known as MPC1 and MPC2 that heteromerize to form a functional complex. It has been reported that MPC2 can also homomerize, however only MPC1/MPC2 heteromeric forms are functional. Complete absence of MPC2 is shown to cause embryonic lethality in murine models, however, murine cell lines that express an N-terminal truncated MPC2 protein (Mpc2 16) are reported to be viable, but exhibit significantly reduced capacity for mitochondrial pyruvate oxidation. (Ref.: Compan, V., et al. (2015). Mol. Cell 59(3); 491-501; Vigueira, PA., et al. (2014). Cell Rep. 7(6); 2042-2053).
Specifications
Mitochondrial Pyruvate Carrier 2 (BRP44) | |
Monoclonal | |
Western Blotting Analysis: 1μg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates. Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland).. Western Blotting Analysis: 4 μg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate. Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056). | |
Mitochondrial pyruvate carrier 2;Brain protein 44;BRP44 | |
Mouse | |
Protein G purified | |
RUO | |
Primary | |
Human, Mouse | |
Purified |
Immunoprecipitation, Western Blot | |
Unconjugated | |
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. | |
MCP2;BRP44 | |
A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein. | |
25 μg | |
Signaling | |
This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region. | |
Stable for 1 year at 2-8°C from date of receipt. | |
IgG1 |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction