Molecular Probes™ MitoProbe™ Transition Pore Assay Kit, for flow cytometry

Catalog No.	Quantity
50-112-1533	1 kit

Catalog No. 50-112-1533 Supplier Molecular Probes™ Supplier No. M34153

Description

MitoProbe™ Transition Pore Assay Kit, for flow cytometry

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Quantity: 1 kit
• Format: Tube
• Product Type: Transition Pore Assay Kit
• Content And Storage: Contains 5 vials of calcein, AM (50 μg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 μg), calcium salt, and 1 vial of DMSO (1.5 mL).
  
  Store in freezer (-5 to -30°C).
• Excitation/Emission: 488
• No. of Reactions: 100 Reactions
• Shipping Condition: Room Temperature
• Product Line: MitoProbe
• Conjugate: Calcein AM

Frequently Asked Questions (FAQs)

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.