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Description
The human HME-MMP12 ELISA kit is for quantitative determination of HME-MMP12 concentrations in human cell culture supernatants and biological fluids (plasma, serum, sputum). This kit is for research purpose only. Kit contents: Capture Antibody: 0.5mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 705D10, DDX0282). Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01, DDX0281). Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A 8 point standard curve using 2-fold serial dilutions in sample dilution buffer, starting at a concentration of 1ug/ml is recommended.
Specifications
Specifications
| Assay Sensitivity | The minimum detectable dose of human HME-MMP12 was determined to be <50pg/mL (Demets et al, 2006) |
| Product Type | ELISA Kit |
| Target Species | Human |
| For Use With (Application) | ELISA |
| Gene ID (Entrez) | 4321 |
| Gene Symbol | MMP12 |
| Kit Contents | Capture Antibody: 0.5mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 705D10), Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01), Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A 8 point standard curve using 2-fold serial dilutions in sample dilution buffer |
| Quantity | 1 Kit |
| Storage Requirements | Storage of components varies. See protocol for specific instructions. |
| Synonym | EC 3.4.24, hME, HMEEC 3.4.24.65, Macrophage elastase, macrophage metalloelastase, matrix metallopeptidase 12 (macrophage elastase), matrix metalloproteinase 12 (macrophage elastase), Matrix metalloproteinase-12, ME, MGC138506, MME, MMP-12 |
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